cd8 t cell detection  (Nature Biotechnology)

Journal: Cells

Article Title: Whole Transcriptome Sequencing Reveals Cancer-Related, Prognostically Significant Transcripts and Tumor-Infiltrating Immunocytes in Mantle Cell Lymphoma

doi: 10.3390/cells11213394

Figure Lengend Snippet: High tumor-infiltrating CD8 + T-cell ratio together with low FTX or PCA3 expression predicts high-risk MCL patients. Kaplan–Meier plots showing the curves based on a combination of tumor-infiltrating CD8 + T-cell ratios and FTX ( A , B ) or PCA3 ( C , D ) transcript expression levels. L/L: Low CD8 + T-cell abundance and low transcript expression; L/H: Low CD8 + T-cell abundance and high transcript expression; H/L: High CD8 + T-cell abundance and low transcript expression; H/H: High CD8 + T-cell abundance and high transcript expression.

Article Snippet: The sections from the FFPE tissues of the cases were stained with CD8 antibody (Anti-CD8 alpha antibody [EPR20305], ready-to-use, Abcam) for CD8 + T-cell detection, or with NCAM1 (Anti-NCAM1 antibody [EPR21827], ready-to-use, Abcam) antibody for NK cell detection using a Ventana Benchmark Ultra system according to the manufacturer’s instructions.

Techniques: Expressing

Journal: BioResearch Open Access

Article Title: Expression of Estrogen-Related Receptors in Localized Provoked Vulvodynia

doi: 10.1089/biores.2019.0049

Figure Lengend Snippet: Examples of ERRα immunostaining showing similar cytoplasmic and nuclear staining in vulvar vestibulum from LPV patients (a1) and healthy controls (a2) . Examples of ERRß immunostaining showing more intense cytoplasmic and nuclear staining in vulvar vestibulum samples from patients with LPV (b1) compared to samples from healthy controls (b2) . Examples of ERRγ immunostaining demonstrating similar nuclear staining in vulvar vestibulum from LPV patients (c1) and healthy controls (c2) . Examples of ER immunostaining showing similar expression pattern between samples from LPV patients (d1) and healthy controls (d2) . Examples of PR immunostaining in vulvar vestibulum samples from LPV patients (e1) and healthy controls (e2) . Examples of CD3 immunostaining identifying both CD4- and CD8-positive T cells in vulvar vestibulum samples from patients with LPV (f1) and healthy controls (f2) . All the histologic sections were visualized with the 10/20 × objective using an Olympus BX-51 light microscope (Tokyo, Japan). ER, estrogen receptor; ERR, estrogen-related receptor; PR, progesterone receptor.

Article Snippet: For CD3 staining, we used anti-CD3e rabbit monoclonal antibody detecting both CD4- and CD8-positive T cells (clone BSR10) at a dilution of 1:200 (Nordic BioSite Ab, Täby, Sweden).

Techniques: Immunostaining, Staining, Expressing, Light Microscopy